G3BP1 (phospho Ser232) rabbit pAb

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

Q13283

Swiss Prot

Q13283

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human G3BP-1 around the phosphorylation site of Ser232. AA range:216-248

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

60kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

60kD

Fragment

IgG

Subcellular Location

Cytoplasm, cytosol . Perikaryon . Cytoplasm, Stress granule . Nucleus . Cytoplasmic in proliferating cells (PubMed:11604510) . Cytosolic and partially nuclear in resting cells (PubMed:11604510) . Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778) . The unphosphorylated form is recruited to stress granules (PubMed:12642610) . HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610) . .