EGFR (phospho Thr693) rabbit pAb

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016],

Product Specifications

Background

Product Name Alternative

EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

UniProt

P00533

Swiss Prot

P00533

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Thr693. AA range:661-710

Target

EGFR

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

175kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

175kD

Fragment

IgG

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane ; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome . Endosome membrane. Nucleus . In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:20674546, PubMed:17909029) . Endocytosed upon activation by ligand (PubMed:2790960, PubMed:17182860, PubMed:27153536, PubMed:17909029) . Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055) . .; [Isoform 2]: Secreted.