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Acetyl eIF5A/eIF5A2 (K47) rabbit pAb

Function: The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond. PTM: eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine). similarity: Belongs to the eIF-5A family. tissue specificity: Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.

Product Specifications

Background

Function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond., PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine) ., similarity:Belongs to the eIF-5A family., tissue specificity:Expressed in ovarian and colorectal cancer cell lines (at protein level) . Highly expressed in testis. Overexpressed in some cancer cells.

Synonyms

EIF5A2; Eukaryotic translation initiation factor 5A-2; eIF-5A-2; eIF-5A2; Eukaryotic initiation factor 5A isoform 2

Gene ID

56648

UniProt

Q9GZV4

Cellular Locus

Cytoplasm. Nucleus. Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side. Nucleus, nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions..

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47. AA range:11-60

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, ELISA

Validated Applications

WB,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES1096-1.pdf

Subcellular Location

Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Nucleus, nuclear pore complex . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. .

Other Product Names

EIF5A2; Eukaryotic translation initiation factor 5A-2; eIF-5A-2; eIF-5A2; Eukaryotic initiation factor 5A isoform 2

Gene ID (Human)

56648

SwissProt (Human)

Q9GZV4

Available Sizes

Curated Selection

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