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Histone H2B (Acetyl Lys5) rabbit pAb

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.

Synonyms

H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s;H2BK5AC

Gene ID

54145

UniProt

P57053

Cellular Locus

Nucleus. Chromosome.

Host

Rabbit

Species Reactivity

Human,Mouse

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys5. AA range:1-50

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

WB, IHC, IF, ELISA

Validated Applications

WB,IHC,IF,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Molecular Weight

18kD

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES1087-1.pdf

Observed Molecular Weight

18 kD

Subcellular Location

Nucleus. Chromosome.

Other Product Names

H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s; H2BK5AC

Gene ID (Human)

54145

SwissProt (Human)

P57053

Available Sizes

Curated Selection

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