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β-actin Rabbit pAb

β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

Product Specifications

Background

β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

Product Name Alternative

Actin antibody, b-actin antibody, beta-actin antibody

UniProt

P60709

Swiss Prot

P60709

Reactivity

Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus

Immunogen

Recombinant Protein

Clonality

Polyclonal

Source

Rabbit

Applications

WB, IHC, IF

Concentration

1mg/mL

Dilution

WB 1:3,000 IHC 1:200 IF 1:200

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Molecular Weight

43kDa

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

43kDa

Fragment

IgG

Subcellular Location

Cytoplasm, Cytoskeleton

Other Product Names

Actin antibody, b-actin antibody, beta-actin antibody

Gene ID (Human)

60

Available Sizes

Curated Selection

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