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β-Tubulin Mouse mAb

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55, 000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

Product Specifications

Background

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

UniProt

P07437

Swiss Prot

P07437

Reactivity

Zebrafish

Immunogen

Synthetic Peptide

Clonality

Monoclonal

Source

Mouse

Applications

WB, IHC, IF

Concentration

1mg/mL

Dilution

WB 1:5,000-20,000 IHC 1:200 IF 1:200

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Molecular Weight

55kDa

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

55kDa

Fragment

IgG1

Gene ID (Human)

203068

Available Sizes

Curated Selection

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