β-actin Mouse mAb
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
Product Specifications
Background
Product Name Alternative
Actin antibody, b-actin antibody, beta-actin antibody
UniProt
P60709
Swiss Prot
P60709
Reactivity
Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep
Immunogen
Synthetic Peptide
Clonality
Monoclonal
Source
Mouse
Applications
WB, IHC, IF
Concentration
1mg/mL
Dilution
WB 1:5,000-10,000 IHC 1:200 IF 1:200
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Molecular Weight
43kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
43kDa
Fragment
IgG1
Subcellular Location
Cytoplasm, Cytoskeleton
Other Product Names
Actin antibody, b-actin antibody, beta-actin antibody
Gene ID (Human)
60
Available Sizes
Frequently Asked Questions
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