His-Tag Mouse mAb
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Product Specifications
Background
Host
Mouse
Immunogen
Synthetic Peptide
Clonality
Monoclonal
Isotype
IgG1
Source
Mouse
Applications
WB, IP, IF
Validated Applications
WB,IP,IF
Stability
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Concentration
1 mg/mL
Dilution
WB 1:5,000 IP 1:200 IF 1:1,000
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.elkbiotech.com/upload/file/Antibodies/mAb/EM1003-1.pdf
Available Sizes
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