Anti-FLIP (CT) Antibody

Rabbit Polyclonal Antibody specific to FLIP (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Caspase homolog, CASH, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, CLARP, Cellular FLICE-like inhibitory protein, c-FLIP, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity, MRIT, Usurpin [Cleaved into: CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12]

Gene Name

CFLAR

Gene ID

8837

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=CFLAR">CFLAR</a>

UniProt

O15519

Accession Number

NP_001120655.1

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 447- 464 at the C-terminus of human FLIPa/FLIPL (accession no. AAC51622)

Target Antigen

CASP8 and FADD-like apoptosis regulator

Target

FLIP (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. {PubMed:9880531}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunocytochemistry: use at 10ug/mL. Positive control: Whole cell lysate from HeLa cells. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length human, mouse, and rat FLIPalpha (55kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Caspase-8 (FLICE) and -10 (FLICE2) are two pivotal members of the ICE/CED-3 protease family. FLICE-inhibitory proteins have been identified in viruses and human cells and are designated v-FLIPs and FLIP, respectively. Human FLIP was cloned by several independent laboratories and designated Casper, I-FLICE, FLAME-1, CASH, and CLARP. FLIP contains two death effector domains and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10 and potently inhibits apoptosis induced by death receptors CD95, DR3, TRAIL-R, and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, g, and delta, respectively.