Human CD30 Ligand / TNFSF8 Protein, His Tag, low endotoxin (MALS verified)
Human CD30 Ligand Protein, His Tag (CDL-H524b) is expressed from human 293 cells (HEK293) . It contains AA Gln 63 - Asp 234 (Accession # P32971-1) .
Product Specifications
Background
CD30 ligand (CD30L) and CD30 are interacting cellsurface glycoproteins that are members of the tumour necrosis factor (TNF) and tumour necrosis factor receptor (TNFR) superfamilies. In humans, CD30L is expressed at high levels on activated T cells. There is conflicting evidence with regard to cell surface expression of CD30L on primary human B cells. An initial report of constitutive CD30L expression on peripheral blood B cells33 has not been substantiated.
Specifications
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.5 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P32971-1
Expression Region
Gln 63 - Asp 234
Host
HEK293
Target
CD30 ligand
Conjugation
Unconjugated
Tag
N-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
0.01 EU per μg
Purity
90%
Bioactivity
Immobilized Human CD30 Protein, Fc Tag (Cat. No. CD0-H5250) at 2 μg/mL (100 μL/well) can bind Human CD30 Ligand Protein, His Tag (Cat. No. CDL-H524b) with a linear range of 0.039-0.313 μg/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
21.5 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
100ug*10
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/CDL-H524b.pdf
Species
Human
Curated Selection
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