Pyruvate Oxidase (purified from microorganisms)

<strong>Pyruvate Oxidase (purified from microorganisms)</strong>_x000D_ <strong>Catalog number:</strong> B2010235_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Volume/Weight:</strong> 1 mg_x000D_ <strong>pH:</strong> na_x000D_ <strong>Supplied as:</strong> Ready-to-use_x000D_ <strong>Appearance:</strong> Lyophilized Powder_x000D_ <strong>Applications:</strong> high purity (95%), high specific activity (10U/mg) dried powder of Pyruvate Oxidase, an enzyme useful for enzymatic determination of pyruvate, GOT and GPT levels in clinical samples._x000D_ <strong>Storage:</strong> -20C_x000D_ <strong>Keywords:</strong> oxygen oxidoreductase_x000D_ <strong>Grade:</strong> Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MŒ©-cm) and are filtered on a 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Lu J, Zhao Y, Zhang J. High-level expression of Aerococcus viridans pyruvate_x000D_ oxidase in Escherichia coli by optimization of vectors and induction conditions._x000D_ Lett Appl Microbiol. 2018 Sep;67(3):262-269._x000D_ _x000D_ 2: Bryant JC, Dabbs RC, Oswalt KL, Brown LR, Rosch JW, Seo KS, Donaldson JR,_x000D_ McDaniel LS, Thornton JA. Pyruvate oxidase of Streptococcus pneumoniae_x000D_ contributes to pneumolysin release. BMC Microbiol. 2016 Nov 9;16(1):271._x000D_ _x000D_ 3: Cornacchione LP, Klein BA, Duncan MJ, Hu LT. Interspecies Inhibition of_x000D_ <i>Porphyromonas gingivalis</i> by Yogurt-Derived <i>Lactobacillus_x000D_ delbrueckii</i> Requires Active Pyruvate Oxidase. Appl Environ Microbiol. 2019_x000D_ Aug 29;85(18):e01271-19._x000D_ _x000D_ 4: Echlin H, Frank MW, Iverson A, Chang TC, Johnson MD, Rock CO, Rosch JW._x000D_ Pyruvate Oxidase as a Critical Link between Metabolism and Capsule Biosynthesis_x000D_ in Streptococcus pneumoniae. PLoS Pathog. 2016 Oct 19;12(10):e1005951._x000D_ _x000D_ 5: Liang J, Zhao J, Wang Z, Wang Y. Temperature gradient-based high-cell density_x000D_ fed-batch fermentation for the production of pyruvate oxidase by recombinant E._x000D_ coli. Prep Biochem Biotechnol. 2018 Feb 7;48(2):188-193._x000D_ _x000D_ 6: Brissac T, Shenoy AT, Patterson LA, Orihuela CJ. Cell Invasion and Pyruvate_x000D_ Oxidase-Derived H₂O₂ Are Critical for Streptococcus_x000D_ pneumoniae-Mediated Cardiomyocyte Killing. Infect Immun. 2017 Dec_x000D_ 19;86(1):e00569-17._x000D_ _x000D_ 7: Taniai H, Iida K, Seki M, Saito M, Shiota S, Nakayama H, Yoshida S. Concerted_x000D_ action of lactate oxidase and pyruvate oxidase in aerobic growth of_x000D_ Streptococcus pneumoniae: role of lactate as an energy source. J Bacteriol. 2008_x000D_ May;190(10):3572-9. doi: 10.1128/JB.01882-07. Epub 2008 Mar 14. PMID: 18344365;_x000D_ PMCID: PMC2395018._x000D_ _x000D_ 8: Carvalho SM, Farshchi Andisi V, Gradstedt H, Neef J, Kuipers OP, Neves AR,_x000D_ Bijlsma JJ. Pyruvate oxidase influences the sugar utilization pattern and_x000D_ capsule production in Streptococcus pneumoniae. PLoS One. 2013 Jul_x000D_ 3;8(7):e68277._x000D_ _x000D_ 9: Goffin P, Muscariello L, Lorquet F, Stukkens A, Prozzi D, Sacco M,_x000D_ Kleerebezem M, Hols P. Involvement of pyruvate_oxidase activity and acetate_x000D_ production in the survival of Lactobacillus plantarum during the stationary_x000D_ phase of aerobic growth. Appl Environ Microbiol. 2006 Dec;72(12):7933-40._x000D_ _x000D_ 10: Belanger AE, Clague MJ, Glass JI, Leblanc DJ. Pyruvate_oxidase is a_x000D_ determinant of Avery's rough morphology. <a href="https://pubmed.ncbi.nlm.nih.gov/15576764/">J Bacteriol.</a> 2004 Dec;186(24):8164-71._x000D_ _x000D_ <strong>Products Related to Pyruvate Oxidase (purified from microorganisms)</strong><a>: Biochemical Buffers and Solutions</a>