Anti-Calnexin (NT) Antibody

Rabbit Polyclonal Antibody specific to Calnexin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

IP90, Major histocompatibility complex class I antigen-binding protein p88, p90

Gene Name

CANX

NCBI Gene ID

UniProt

P27824

Cellular Locus

Endoplasmic reticulum membrane, Endoplasmic reticulum, Melanosome

Host

Rabbit

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Pig, Monkey, Rabbit, Sheep

Immunogen

A 19 amino acid synthetic peptide corresponding to canine calnexin conjugated to KLH.

Target Antigen

Calnexin

Target

Calnexin (NT)

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Neuroscience

Purification Method

Whole antiserum

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Whole Antiserum

Form

Liquid

Buffer

Whole Antiserum

Function

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

Additionnal Information

Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of ~90 kDa is detected. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application. <br><br>Positive control: HeLa cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, pig, monkey, rabbit, and sheep calnexin.

Formulation

Whole antiserum

Target Background

Calnexin is a ~90 kDa integral protein of the endoplasmic reticulum; it is also referred to as IP90, p88 and p90. It consists of a 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix, and a short acidic cytoplasmic tail. Its positively charged cytosolic residues ensure its retention in the endoplasmic reticulum. Calnexin together with calreticulin play a key role in glycoprotein folding by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor, and B cell surface immuno- globulin.