Dexamethasone ELISA Kit

Dexamethasone is a synthetic glucocorticoid that is used to treat inflammatory conditions such as allergies, arthritis, lupus, and ulcerative colitis, numerous skin diseases, blood, eye, and kidney disorders. It is also given to patients undergoing chemotherapy to neutralize the side effects of antitumor treatments. Dexamethasone when combined with certain foods may cause drug-food interaction. Grapefruit and pomegranate juices increase the concentration of dexamethasone in the blood by interfering with the ability of hepatic enzymes to clear the drug from the body. Consumption of alcohol with dexamethasone may cause stomach ulcers. BioVision’s Dexamethasone ELISA kit is used to quantitatively measure Dexamethasone in muscle tissue, milk, and feed samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Dexamethasone antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Dexamethasone in the samples.