Human LGR4 / GPR48 protein, His Tag

The fourth member of the leucine-rich repeat-containing GPCR family (LGR4, frequently referred to as GPR48) together with its family members LGR5 and -6, bind to R-spondins (RSPOs) -1–4 and result in Wnt signaling potentiation. In addition, LGR4 (as well as LGR5 and -6) is implicated in multiple cancers and promotes invasion and metastasis in colorectal, prostate, and cervical cancer cell lines.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.8 kDa. The protein migrates as 70-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q9BXB1-1

Expression Region

Ala 25 - Thr 544

Host

HEK293

Target

LGR4

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

85%

Bioactivity

Immobilized Human R‑Spondin 3 at 2 μg/mL (100 μL/well) can bind Human LGR4, His Tag (Cat. No. LG4-H52H3) with a linear range of 10-78 ng/mL (QC tested).