Vanillylmandelic Acid ELISA Kit

Vanillylmandelic acid (VMA), a metabolic by-product of norepinephrine and epinephrine, can be used to detect neuroblastoma and other tumors of neural crest origin. BioVision’s Vanillylmandelic acid ELISA kit is based on the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VMA. During the reaction, VMA in the sample or standard competes with a fixed amount of VMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VMA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VMA in the samples is then determined by comparing the OD of the samples to the standard curve.