Deoxynivalenol ELISA Kit

Deoxynivalenol (DON) also known as Vomitoxin is a type B trichothecene, an epoxy-sesquiterpenoid. This mycotoxin occurs predominantly in grains such as wheat, barley, oats, rye, and corn, and less often in rice, sorghum, and triticale. In higher levels and more advanced stages of contamination, the symptoms linked to vomitoxin are much more severe and include neural problems, immune system deficiencies, hemorrhaging and necrosis of the digestive system, lack of blood production in the spleen and bone marrow, and possible birth defects. BioVision’s ELISA Kit is based on Indirect-Competitive-ELISA method. It can detect Deoxynivalenol in samples, such as rice, millet, flour and other crops/feed, etc. The micro-plate provided in this kit has been pre-coated with DON. During the reaction, DON in the samples or standard competes with DON on the solid phase supporter for sites of DON antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of DON. The concentration of DON in the samples can be calculated by comparing the OD of the samples to the standard curve.