MMP-2 (Rat) ELISA Kit

The mammalian Matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. After cleavage of a single peptide domain of about 20 amino acids, the MMPs are secreted in latent forms. Upon activation, the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-2 (72 kDa type IV collagenase, Gelatinase-A) contains the basic structure of propeptide, catalytic, and hemopexin domains. It is an important proteinase in tissue remodeling. BioVision’s MMP-2 ELISA kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to rat MMP-2. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for rat MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat MMP-2.The concentration of Rat MMP-2 in the samples was calculated by comparing the OD of the samples to the standard curve.