cAMP ELISA Kit

Cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) are second messenger molecules important in regulating intracellular energy metabolism via actions on specific protein kinases. Both are highly enriched in the CNS; cAMP is involved in higher cortical functions, while cGMP plays a role in phototransduction. Most studies suggest that normal lumbar CSF (Cerebrospinal fluid) in adults contains 15–30 nmol/l of cAMP and one study reports that intraventricular levels may be 2- to 3-fold than lumbar levels. cAMP is a derivative of adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms, conveying the cAMP-dependent pathway. cAMP involvement in mechanisms of transmembrane regulation of cell metabolism, differentiation, proliferation in malignant growth is promising as protecting the CT and regulating body homeostasis. This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in the sample or standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.