3’ to 5’ Exonuclease Activity Assay (Fluorometric)

Exonucleases remove a single nucleotide or an oligonucleotide at the 5’ or 3’ end. Exonuclease, such as Three Prime Repair Exonuclease 1 (TREX1), plays an important role in proofreading of DNA amplification and removal of damaged DNA. Deficiency of TREX1 enzyme has been shown to trigger autoimmune disease, Aicardia-Goutieres syndrome. TREX1 expression has been reported to correlate with cervical cancer cell growth and disease progression. In addition, 3’ exonucleases are often used as tools in molecular biology such as site-directed mutagenesis and the generation of single stranded DNA probe. BioVision’s 3’ to 5’ Exonuclease Activity Assay Kit provides a quick and easy method for monitoring 3’ exonuclease activities in a wide variety of samples. In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (Ex/Em= 304/369nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.2 µU of exonuclease activity.