Chlamydia Trachomatis IgM ELISA Kit

Chlamydia Trachomatis is one of the most common human pathogens. Of the 15 recognized serotypes, 4 (A, B, Ba, and C) have been shown to cause hyperendemic blinding trachoma, a disease which afflicts hundreds of millions of people in developing countries. Three serotypes (L-1, L-2, and L-3) are the causes of lymphogranuloma venereum (LGV), a sexually transmitted systemic disease. BioVision's Chlamydia Trachomatis ELISA Kit can detect LGV type 2 broadly reacting antigen of Chlamydia Trachomatis. It will detect Chlamydia Trachomatis, Chlamydia Psittaci and Chlamydia Pneumoniae (TWAR) antibodies. Purified Chlamydia Trachomatis antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the Chlamydia Trachomatis IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off, and TMB Chromogenic Substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.