Anti-Copper-Transporting ATPase2 Antibody

Mouse Monoclonal Antibody specific to Copper-Transporting ATPase2

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Copper-Transporting ATPase2 Mouse Monoclonal Antibody

Gene Name

ATP7B

NCBI Gene ID

UniProt

B7ZLR4

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide correspond- ing to aa 3-21 (cytoplasmic N-terminus) of human Copper-Transporting ATPase2. (accession no. NP_000044.2).

Target Antigen

P-type Cu(+) transporter

Target

Copper-Transporting ATPase2

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~160kDa is detected. Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat Copper-Transporting ATPase2.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from cells. ATP7b (also known as Copper-Transporting ATPase2) transports copper in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene: A is found in the liver, kidney and brain, the shorter form B is found in brain, and the third isoform, known as WND/140KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.