Recombinant Mycobacterium tuberculosis Metallothionein (mymT)
Product Specifications
Product Name Alternative
MT; Copper-binding metallothionein; CuI; Mycobacterial metallothionein
Abbreviation
Recombinant Mycobacterium tuberculosis mymT protein
Gene Name
MymT
UniProt
P9WK09
Expression Region
7-53aa
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Target Sequence
TNYEAGTLLTCSHEGCGCRVRIEVPCHCAGAGDAYRCTCGDELAPVK
Tag
C-terminal 6xHis-tagged
Type
In Stock Protein
Source
Yeast
Field of Research
Epigenetics and Nuclear Signaling
Relevance
Metallothioneins are small proteins that have a high content of cysteine residues wich allow them to bind heavy metal ions through clusters of thiolate bonds. MymT binds up to seven ions of Cu (+), with a preference for four to six Cu (+) ions, in a solvent-shielded core. MymT protects M.tuberculosis from copper toxicity.
Endotoxin
Not test
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
6.4 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Product MSDS
https://www.cusabio.com/msds/2937012/
Protein Length
Full Length of Mature Protein
Available Sizes
Curated Selection
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