Anti-SHPRH Antibody

Rabbit Polyclonal Antibody specific to SHPRH Antibody

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.3.2.27, EC 3.6.4.-, RING-type E3 ubiquitin transferase SHPRH, SNF2, histone-linker, PHD and RING finger domain-containing helicase

Gene Name

SHPRH

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=SHPRH">SHPRH</a>

UniProt

Q149N8

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa residues 515-535 (DKTKKQAVGSPRKIEKELRKS) of mouse SHPRH conjugated to KLH at the N-terminus.

Target Antigen

E3 ubiquitin-protein ligase SHPRH

Target

SHPRH

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Cancer research

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. {PubMed:17108083, PubMed:17130289, PubMed:18719106}.

Additionnal Information

Immunoblotting : use at 1:250 dilution. <br><br>Positive control: Mouse brain lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes mouse SHPRH (approx. 185 kDa) which has 90% sequence homology with human and 95% sequence homology with rat.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A novel gene containing SNF2, PHD- finger, RING-finger, helicase, and linker histone domains termed SHPRH, is a possible candidate for a tumor suppressor gene. These domains are characteristic of several DNA repair proteins, transcription factors, and helicases. Mapping of this gene to 6q24, a region reported to contain a tumor suppressor locus, suggests a role for SHPRH in tumor suppression.