Anti-T7 Antibody, HRP conjugate

Goat Polyclonal Antibody specific to T7 HRP

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-T7 HRP Goat Polyclonal Antibody

Host

Goat

Reactivity

Immunogen

T7 (MASMTGGQQMG) conjugated to KLH.

Target

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Epitope Tags

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000- 1:30,000. Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000- 1:100,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at 2-8°C.

Specificity

Antibody was affinity-purified on the peptide.

Formulation

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400

Buffer pH

pH 7.2

Target Background

The T7 tag is an 11 amino acid peptide (MASMTGGQQMG) derived from the leader sequence of T7 bacteriophage gene10 which encodes a T7 major capsid protein. The T7 tag serves as a tag in many expression vectors, and antibodies specific for the T7 tag are useful for identifying and isolating T7-tagged proteins.