5-CFDA-AM
Product Specifications
UNSPSC Description
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].
Target Antigen
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/5-cfda-am.html
Purity
99.81
Solubility
DMSO : 125 mg/mL (ultrasonic)
Smiles
O=C(C1=CC2=C(C3(C4=C(OC5=C3C=CC(OC(C)=O)=C5)C=C(OC(C)=O)C=C4)OC2=O)C=C1)OCOC(C)=O
Molecular Weight
532.45
References & Citations
[1]Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89(3):216-21.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, protect from light)
Product Datasheet
http://file.medchemexpress.com/batch_PDF/HY-131131/5-CFDA-AM-DataSheet-MedChemExpress.pdf
Product MSDS
http://file.medchemexpress.com/batch_PDF/HY-131131/5-CFDA-AM-SDS-MedChemExpress.pdf
Clinical Information
No Development Reported
CAS Number
124412-00-6
Available Sizes
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