Anti-BACE (CT) Antibody

Rabbit Polyclonal Antibody specific to BACE (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.4.23.46, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2

Gene Name

BACE1

Gene ID

23621

NCBI Gene ID

UniProt

P56817

Accession Number

NP_620428

Cellular Locus

Cell membrane, Golgi apparatus, trans-Golgi network, Endoplasmic reticulum, Endosome, Cell surface, Cytoplasmic vesicle membrane, Membrane raft, Lysosome, Late endosome, Early endosome, Recycling endosome, Cell projection, axon, Cell projection, dendrite

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Peptide corresponding to aa 485-501 of human BACE. This sequence differs from those of mouse and rat by one amino acid.

Target Antigen

Beta-secretase 1

Target

BACE (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IHC-P, IF

Field of Research

Neuroscience

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (PubMed:10656250, PubMed:10677483, PubMed:20354142). Cleaves CHL1 (By similarity). {UniProtKB:P56818, PubMed:10656250, PubMed:10677483, PubMed:20354142}.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution. <br><br>Positive control: Human brain tissue lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human and mouse BACE (approx. 70 kD).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated as a result of proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and g-sites by two proteases. APP is first cleaved by beta- secretase to produce a soluble derivative of the protein and a membrane-anchored 99- amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kD amyloid-beta peptide which is found in brains of patients with Alzheimer's disease. beta-secretase was recently identified and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a transmembrane aspartic protease and co- localizes with APP.