Chk2 (Phospho Thr432) rabbit pAb

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Disease: Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM: 609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. Disease: Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM: 259500]. Disease: Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM: 176807]. enzyme regulation: Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation. function: Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. similarity: Contains 1 FHA domain. similarity: Contains 1 protein kinase domain. subcellular location: Isoform 10 is present throughout the cell. tissue specificity: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.