Anti-BRCA1 Antibody

Mouse Monoclonal Antibody specific to BRCA1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.3.2.27, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1

Gene Name

BRCA1

Gene ID

672

NCBI Gene ID

UniProt

P38398

Accession Number

NP_009225

Cellular Locus

Nucleus, Chromosome, Cytoplasm

Host

Mouse

Reactivity

Human

Immunogen

GST fusion protein expressed in E. coli corresponding to aa 762-1315 of full-length BRCA1.

Target Antigen

Breast cancer type 1 susceptibility protein

Target

BRCA1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC-P, IP, IHC

Field of Research

Cancer Research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage (PubMed:12890688, PubMed:14976165, PubMed:16818604, PubMed:17525340, PubMed:12887909, PubMed:10500182, PubMed:19261748). It is unclear whether it also mediates the formation of other types of polyubiquitin chains (PubMed:12890688). The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability (PubMed:12890688, PubMed:14976165, PubMed:20351172). Regulates centrosomal microtubule nucleation (PubMed:18056443). Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle (PubMed:10724175, PubMed:12183412, PubMed:11836499, PubMed:19261748). Required for FANCD2 targeting to sites of DNA damage (PubMed:12887909). Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation (PubMed:16326698). Contributes to homologous rPubMed:10500182, PubMed:10724175, PubMed:11836499, PubMed:12183412, PubMed:12887909, PubMed:12890688, PubMed:14976165, PubMed:16326698, PubMed:16818604, PubMed:17525340, PubMed:18056443, PubMed:19261748, PubMed:19369211, PubMed:20160719, PubMed:20351172}.

Additionnal Information

Immunoblotting: use at 1-10 ug/mL. Immunoprecipitation: use at 1-10 ug/mL. Immunohistochemistry: use at 1-10 ug/mL. Positive controls: Any normal human tissue, especially those containing rapidly proliferating cells, such as lymphoid germinal centers.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length BRCA1, a 220 kD nuclear phosphoprotein, and does not recognize the deltaexon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.