IMAC Buffer (pH 8.0)

<strong>IMAC Buffer (pH 8.0)</strong>_x000D_ <strong>Catalog number:</strong> B2014673_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 250 mL_x000D_ <strong>Molecular Weight or Concentration:</strong> N/A_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> 2-8°C_x000D_ <strong>Keywords:</strong> IMAC Buffer (pH 8.0)_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Martins S, Andrade J, Karmali A, Serralheiro ML. Screening of suitable immobilized metal chelates for adsorption of monoclonal antibodies against mutant amidase from Pseudomonas aeruginosa J Mol Recognit. 2006 Jul-Aug;19(4):340-7._x000D_ 2: Belew M, Yip TT, Andersson L, Ehrnström R. High-performance analytical applications of immobilized metal ion affinity chromatography Anal Biochem. 1987 Aug 1;164(2):457-65._x000D_ 3: Martins S, Karmali A, Andrade J, Serralheiro ML. Immobilized metal affinity chromatography of monoclonal immunoglobulin M against mutant amidase from Pseudomonas aeruginosa Mol Biotechnol. 2006 Jun;33(2):103-14._x000D_ 4: Zachariou M, Hearn MT. Adsorption and selectivity characteristics of several human serum proteins with immobilised hard Lewis metal ion-chelate adsorbents J Chromatogr A. 2000 Aug 18;890(1):95-116._x000D_ 5: Zachariou M, Hearn MT. Protein selectivity in immobilized metal affinity chromatography based on the surface accessibility of aspartic and glutamic acid residues J Protein Chem. 1995 Aug;14(6):419-30._x000D_ 6: do Rosário Freixo M, Karmali A, Arteiro JM. Production of polygalacturonase from Coriolus versicolor grown on tomato pomace and its chromatographic behaviour on immobilized metal chelates J Ind Microbiol Biotechnol. 2008 Jun;35(6):475-84._x000D_ 7: Huang Q, Chen L, Zeng L, Wan L, Li S, Lu X, Cheng J. [Evaluation of three in-vitro refolding methods for human-derived anti-CTLA4 scFv expressed in E. coli] Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2006 Apr;23(2):388-91._x000D_ 8: Thingholm TE, Jensen ON. Enrichment and characterization of phosphopeptides by immobilized metal affinity chromatography (IMAC) and mass spectrometry Methods Mol Biol. 2009;527:47-56, xi._x000D_ 9: Zhang A, Zhang C, Warikoo V, Forstrom J, Riske F. A modified IMAC method for the capture of target protein from mammalian cell culture harvest containing metal chelating species Biotechnol Bioeng. 2012 Mar;109(3):747-53._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/16294313">10: Barnouin KN, Hart SR, Thompson AJ, Okuyama M, Waterfield M, Cramer R. Enhanced phosphopeptide isolation by Fe(III)-IMAC using 1,1,1,3,3,3-hexafluoroisopropanol Proteomics. 2005 Nov;5(17):4376-88. </a>_x000D_ _x000D_ <strong>Products Related to IMAC Buffer (pH 8.0) can be found at</strong> <a href="https://moleculardepot.com/product-category/Chemicals/"> Chemicals</a>