Anti-Glutathione-S-Transferase (GST) Antibody

Mouse Monoclonal Antibody specific to GST

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GST 28

UniProt

P26624

Host

Mouse

Reactivity

Glutathione-S-Transferase (Gst)

Immunogen

GST protein of Schistosoma japonicum expressed in E. coli.

Target Antigen

Glutathione S-transferase class-mu 28 kDa isozyme

Target

Glutathione-S-Transferase (GST)

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IP

Field of Research

Epitope Tags

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

Additionnal Information

Immunoblotting: use at 0.2-2ug/mL. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes GST.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.