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Human CXCL9 Protein, His Tag

Human CXCL9 Protein, His Tag (CX9-H51H3) is expressed from E. coli cells. It contains AA Thr 23 - Thr 125 (Accession # Q07325).

Product Specifications

Background

CXCL9 is a small cytokine belonging to the CXC chemokine family that is also known as monokine induced by gamma interferon (MIG). CXCL9 is a chemokine that plays a role in immune responses, it can induce chemotaxis, promote differentiation and proliferation of leukocytes, and cause tissue extravasation. CXCL9 regulates immune cell migration, differentiation and activation by binding to CXCR3 receptor, such as CTLs, NK cells, NKT cells and macrophages. CXCL9 also participates in Th1 polarization and IFN-γ response.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.7 kDa. The protein migrates as 17-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Expression Region

Thr 23 - Thr 125

Host

E. coli

Target

CXCL9

Conjugation

Unconjugated

Tag

C-10×His

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human CXCL9 Protein, His Tag (Cat. No. CX9-H51H3) at 5 μg/mL (100 μL/well) can bind Human CXCR3 Protein, Flag,His Tag (Cat. No. CX3-H52D5) with a linear range of 0.019-0.313 μg/mL (QC tested).

Format

Powder

Buffer

PBS, 0.5 M NaCl, 0.5 M Arginine, pH7.4

Additives

Trehalose

Molecular Weight

20.7 kDa

Shipping Conditions

Room Temperature

Storage Conditions

-20°C

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