Anti-Apaf1 (NT) Antibody

Rabbit Polyclonal Antibody specific to Apaf1 (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

APAF-1

Gene Name

APAF1

Gene ID

317

NCBI Gene ID

UniProt

O14727

Accession Number

NP_001151

Cellular Locus

Cytoplasm

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Peptide corresponding to aa 12-28 of human Apaf1. This sequence is identical in human and mouse.

Target Antigen

Apoptotic protease-activating factor 1

Target

Apaf1 (NT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. {PubMed:10393175, PubMed:12804598}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL Immunohistochemistry: use at 1-2ug/mL These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Positive control: Whole cell lysate of HeLa or K562 cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length mouse and human Apaf1 (115-130kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease- activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.