Recombinant Human Laminin511 E8 Protein
Product Specifications
Product Name Alternative
Laminin 511, LN511
UniProt
O15230&P07942&P11047
Expression Region
2534-3323&1561-1786&1364-1609
Host
Mammalian Cells
Tag
N-terminal His & N-terminal Flag & N-terminal HA Tag
Applications
Western Blot, ELISA
Endotoxin
< 0.01 EU/µg as determined by LAL test.
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Measured by the ability of the immobilized protein to support the adhesion of H9 Human embryonic stem cells. The ED50 for this effect is 0.02017 μg/mL
Form
Lyophilized powder
Reconstitution
Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
32-36&36-49&100-120 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from 0.2 μm filtered solution of PBS, pH 7.4.
Organism Species
Human
Available Sizes
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