SingleFlowEx CD22 PerCP-Cy™5.5

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

S-HCL-1

Applications

FC (QC tested)

Concentration

0.060 mg/mL

Format

PerCP-Cy™5.5

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Reineks EZ, Osei ES, Rosenberg A, Auletta J, Meyerson HJ: CD22 expression on blastic plasmacytoid dendritic cell neoplasms and reactivity of anti-CD22 antibodies to peripheral blood dendritic cells. Cytometry B Clin Cytom. 2009 Jul;76 (4) :237-48, URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=Reineks+EZ%2C+Osei+ES%2C+Rosenberg+A , *James SE, Greenberg PD, Jensen MC, Lin Y, Wang J, Till BG, Raubitschek AA, Forman SJ, Press OW: Antigen sensitivity of CD22-specific chimeric TCR is modulated by target epitope distance from the cell membrane. J Immunol. 2008 May 15;180 (10) :7028-38., URL: http://www.ncbi.nlm.nih.gov/pubmed/18453625 , *Huang J, Fan G, Zhong Y, Gatter K, Braziel R, Gross G, Bakke A: Diagnostic usefulness of aberrant CD22 expression in differentiating neoplastic cells of B-Cell chronic lymphoproliferative disorders from admixed benign B cells in four-color multiparameter flow cytometry. Am J Clin Pathol. 2005 Jun;123 (6) :826-32., URL: http://www.ncbi.nlm.nih.gov/pubmed/15899772 , *Foon KA, Todd RF 3rd: Immunologic classification of leukemia and lymphoma. Blood. 1986 Jul;68 (1) :1-31., URL: http://www.ncbi.nlm.nih.gov/pubmed/2941082, *Use of this product has not been published., URL:

Other References

*Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7, URL: https://www.ncbi.nlm.nih.gov/pubmed/18067554?ordin, *Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol. 2007 Aug;27 (16) :5699-710., URL: https://www.ncbi.nlm.nih.gov/pubmed/17562860?ordin, *Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50. , URL: https://www.ncbi.nlm.nih.gov/pubmed/16227086?ordin