Phospho-Smad1/5/9 (S463/S465/S467) Recombinant Rabbit Monoclonal Antibody [JE59-46]
Selected Variant
HA722566
Size: 100 µL
Smads (or SMADs) comprise a family of structurally similar proteins that are the main signal transducers for receptors of the transforming growth factor beta (TGF-B) superfamily, which are critically important for regulating cell development and growth. There are three distinct sub-types of Smads: receptor-regulated Smads (R-Smads), common partner Smads (Co-Smads), and inhibitory Smads (I-Smads). The eight members of the Smad family are divided among these three groups. Trimers of two receptor-regulated SMADs and one co-SMAD act as transcription factors that regulate the expression of certain genes. The R-Smads consist of Smad1, Smad2, Smad3, Smad5 and Smad8/9, and are involved in direct signaling from the TGF-B receptor. R/Co-Smads are primarily located in the cytoplasm, but accumulate in the nucleus following TGF-β signaling, where they can bind to DNA and regulate transcription. However, I-Smads are predominantly found in the nucleus, where they can act as direct transcriptional regulators.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
SMAD1, BSP1, MADH1, MADR1, SMAD5, MADH5, SMAD9, MADH6, MADH9, SMAD8
Swiss Prot
Q15797 Human, Q99717 Human, O15198 Human, P70340 Mouse, P97454 Mouse, Q9JIW5 Mouse, O54835 Rat, P97588 Rat, Q9R1V3 Rat
Cellular Locus
Cytoplasm, Nucleus, Mitochondrion.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser463/465 of human SMAD1 and SMAD5 protein.
Isotype
IgG
Clone
JE59-46
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC, IF-Cell
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IHC: 1:1,000 ;IF-Cell: 1:100
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 52 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Price
Frequently Asked Questions
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