5- (Biotinamido) pentylamine

5- (Biotinamido) pentylamine is an amine donor substrate for transglutaminase. 5- (Biotinamido) pentylamine can be used as a biotin-labeled probe to specifically participate in the calcium-dependent reaction catalyzed by TG, bind to the γ-carboxamide group of the glutamine residue in the protein, introduce the biotin tag into the target protein, and form a biotinylated protein product. 5- (Biotinamido) pentylamine can be used for the labeling, separation, and detection of TG amine receptor protein substrates[1][2][3].

Product Specifications

CAS Number

[115416-38-1]

UNSPSC

12352005

Hazard Statement

H315, H319

Target

Transglutaminase

Type

Reference compound

Related Pathways

Metabolic Enzyme/Protease

Applications

Cancer-programmed cell death

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/5-biotinamido-pentylamine.html

Purity

99.96

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O=C(NCCCCCN)CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O

Molecular Formula

C15H28N4O2S

Molecular Weight

328.47

Precautions

H315, H319

References & Citations

[1]Ikura K, et al. Identification of amine acceptor protein substrates of transglutaminase in liver extracts: use of 5- (biotinamido) pentylamine as a probe. Arch Biochem Biophys. 1998 Aug 15;356 (2) :280-6.|[2]Singh RN, et al. Detection of the amine acceptor protein substrates of transglutaminase with 5- (biotinamido) pentylamine. Anal Biochem. 1995 Oct 10;231 (1) :261-3.|[3]Ichikawa A, et al. Identification of new amine acceptor protein substrate candidates of transglutaminase in rat liver extract: use of 5- (biotinamido) pentylamine as a probe. Biosci Biotechnol Biochem. 2008 Apr;72 (4) :1056-62.