LC3 A/B rabbit pAb

Function: Probably involved in formation of autophagosomal vacuoles (autophagosomes). PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. similarity: Belongs to the MAP1 LC3 family. subcellular location: LC3-II binds to the autophagic membranes. subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. tissue specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

Product Specifications

Background

Function:Probably involved in formation of autophagosomal vacuoles (autophagosomes) ., PTM:The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II., similarity:Belongs to the MAP1 LC3 family., subcellular location:LC3-II binds to the autophagic membranes., subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins., tissue specificity:Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

UniProt

Q9H492/Q9GZQ8

Swiss Prot

Q9H492/Q9GZQ8

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized peptide derived from human LC3 A/B

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA; IHC

Concentration

1 mg/ml

Dilution

WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

Molecular Weight

14,16kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

14,16kD

Fragment

IgG

Subcellular Location

Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Cytoplasm, cytoskeleton . LC3-II binds to the autophagic membranes. .