Recombinant Human ALPP Protein (His Tag)

Product Specifications

Background

Most importantly, placental alkaline phosphatase (ALPP), an ectoenzyme that locates on cell surface with catalytic domains outside the plasma membrane and is overexpressed on many cancer cells, dephosphorylate the d-tyrosine phosphates on the surface of the magnetic nanoparticle and enable the magnetic nanoparticles to adhere selectively to the cancer cells, such as HeLa cells. Placental alkaline phosphatase (PLAP), encoded by the ALPP gene, is produced by the fetal side of the placenta.

Abbreviation

ALPP

Synonyms

Placental alkaline phosphatase; ALPP; ALP; PALP; PLAP; PLAP-1; alkaline phosphatase; placental; ALPP and PLAP; placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; alkaline phosphatase; ALP; PALP; placental; PLAP; PLAP-1; Alkaline phosphatase placental; Alkaline phosphatase placental type; Alp1; FLJ61142; Germ-cell alkaline phosphatase; nagao isozyme; OTTHUMP00000164354; placental heat-stable alkaline phosphatase; PLAP1; PPB1

UniProt

P05187

Accession Number

P05187

Expression System

Mammalian

Tag

C-His

Sequence

Ile23-Asp506

Field of Research

Cell Biology; Metabolism

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.