Recombinant Human ALPP Protein (His Tag)
Product Specifications
Background
Most importantly, placental alkaline phosphatase (ALPP), an ectoenzyme that locates on cell surface with catalytic domains outside the plasma membrane and is overexpressed on many cancer cells, dephosphorylate the d-tyrosine phosphates on the surface of the magnetic nanoparticle and enable the magnetic nanoparticles to adhere selectively to the cancer cells, such as HeLa cells. Placental alkaline phosphatase (PLAP), encoded by the ALPP gene, is produced by the fetal side of the placenta.
Abbreviation
ALPP
Synonyms
Placental alkaline phosphatase; ALPP; ALP; PALP; PLAP; PLAP-1; alkaline phosphatase; placental; ALPP and PLAP; placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; alkaline phosphatase; ALP; PALP; placental; PLAP; PLAP-1; Alkaline phosphatase placental; Alkaline phosphatase placental type; Alp1; FLJ61142; Germ-cell alkaline phosphatase; nagao isozyme; OTTHUMP00000164354; placental heat-stable alkaline phosphatase; PLAP1; PPB1
UniProt
P05187
Accession Number
P05187
Expression System
Mammalian
Tag
C-His
Sequence
Ile23-Asp506
Field of Research
Cell Biology; Metabolism
Endotoxin
< 1.0 EU/mg of the protein as determined by the LAL method
Purity
> 95% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
53.1 kDa
Observed Molecular Weight
60-70 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
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