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Recombinant Peptidase T (pepT)

Product Specifications

Product Name Alternative

(Aminotripeptidase) (Tripeptidase) (Tripeptide aminopeptidase)

Abbreviation

Recombinant Clostridium perfringens pepT protein

Gene Name

PepT

UniProt

Q8XPD8

Expression Region

1-406aa

Organism

Clostridium perfringens (strain 13 / Type A)

Target Sequence

MKKVHERFLEYVKVDTKSDETTRVTPSTKGQLELGKMLAEELKEIGVDEVRISEEGYVYACLKSNCNKDIPKIGFISHMDTAPDMSGKNVNPKIVENYDGKDIELGNGYTLSPSFSPELPMYKGQTLITTDGTTLLGADDKAGIAEIVTAIEYLINHPEIKHGDIKIGFTPDEEIGEGADHFDVEGFGADFAYTLDGGRIGELEYENFNAASAKVEIIGKNVHPGSAKGKMINSILVAHEFVSMLPLDEVPEKTEGYEGFSFLLDIQGEVEKTSLSFIIRDFDKEGFKNRKERFNEIAKELNKKYGEGTVTVTLKDQYMNMKEMIEPRMHIVETAEKAMKQCGIEPIKNPIRGGTDGARLSFMGLPTPNLFTGGENFHGRYEYISINSMEKAVEVILNIIKIYAEK

Tag

C-terminal 6xHis-tagged

Type

In Stock Protein

Source

Yeast

Field of Research

Others

Relevance

Cleaves the N-terminal amino acid of tripeptides.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

46.3 kDa

References & Citations

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001 (2002)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length

Available Sizes

Frequently Asked Questions

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