Amantadine ELISA Kit

Amantadine is an antiviral medication that is used to treat dyskinesia, a movement disorder caused by Parkinson's disease. Although the mechanism is not clearly understood, it is known to act as a dopamine agonist and antagonist for the NMDA receptor. Due to mild side effects on the central nervous system, it is recommended not to combine Amantadine with other CNS stimulants or anticholinergic drugs. Additionally, it is also used to treat influenza A infection. Amantadine blocks the influenza A M2 ion channel and thereby inhibits the replication of the virus. BioVision’s Amantadine ELISA kit is used to quantitatively measure Amantadine in samples such as muscle tissue, milk, and eggs. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Amantadine antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Amantadine in the samples.