Recombinant Human coronavirus HKU1 Protein I (N)

Product Specifications

Product Name Alternative

Accessory protein N2; N internal ORF protein; IORF; Orf8 protein; Protein in nucleocapsid ORF

Abbreviation

Recombinant Human coronavirus HKU1 protein I (N) protein

Gene Name

UniProt

Q5MQC5

Expression Region

1-205aa

Organism

Human coronavirus HKU1 (isolate N1) (HCoV-HKU1)

Target Sequence

MLEVEAPLEIVQESSRKLLGLTNLSEITKPLIEAEKPNLNSLCLLNHKEILSHIIPGSPGSLNFKKVETLNFQMVKEFPLLSEYPLLKQKDIGIDTAGVLLKQLMVNKSSCYRDGISTISVPAHMPMHPMVNPSKGSSGLLITKLTLLLPPMFRQGILLLKKLSLLGFRLVRFCLKAIMLKAQEGLLLIVDQVHVLNHVDPIIVH

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Structural protein that is not essential for the viral replication either in tissue culture or in its natural host.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

27.8 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.