Safranin

Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. Safranin can readily intercalate into biological macromolecules, including DNA and proteins. Safranin has antibacterial effects against gram-positive bacteria (S. aureus) . Safranin can be used as a redox indicator in the determination of metal ion concentration[1][2][3][4].

Product Specifications

CAS Number

[477-73-6]

Product Name Alternative

Safranine T

UNSPSC

12171500

Hazard Statement

H318

Target

Bacterial; Fluorescent Dye

Type

Dye Reagents

Related Pathways

Anti-infection; Others

Field of Research

Infection; Metabolic Disease

Assay Protocol

https://www.medchemexpress.com/safranin.html

Solubility

DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CC1=CC2=NC3=C([N+](C4=CC=CC=C4)=C2C=C1N)C=C(N)C(C)=C3.[Cl-]

Molecular Formula

C20H19ClN4

Molecular Weight

350.85

Precautions

H318

References & Citations

[1]Wan H, et al. Structure characterization and optical properties investigation of the four main components of the classical phenazinium dye Safranin O. Analyst. 2019;144 (24) :7149-7156. |[2]Swain D, et al. Differential staining of the cell cycle of plant cells using safranin and indigo-picrocarmine. Stain Technol. 1990;65 (4) :197-204.|[3]Krumschnabel G, et al. Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol. 2014;542:163-81.|[4]Al-Khikani F, et al. The Antibacterial Action of Safranin and Gentian Violet. Rambam Maimonides Med J. 2022 Jul 31;13 (3) :e0018.