Diazepam ELISA Kit

Diazepam is mainly used to treat anxiety, insomnia, panic attacks and symptoms of acute alcohol withdrawal. It is also used as a premedication for inducing sedation, anxiolysis, or amnesia before certain medical procedures (e.g., endoscopy). Diazepam is the drug of choice for treating benzodiazepine dependence with its long half-life allowing easier dose reduction. It is frequently employed in animal production for its excellent growth promotion properties. However, in humans, an overdose of diazepam leads to severe side effects. This has led to a prohibition of diazepam for the treatment of animals used for food production. BioVision’s Diazepam ELISA Kit is based on Competitive ELISA method. It can detect Diazepam in Tissue, Urine and Feed samples. The microtiter plate provided in the kit has been pre-coated with Diazepam. During the reaction, Diazepam in the samples or standard competes with coated Diazepam for anti- Diazepam antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of Diazepam. The concentration of Diazepam in the samples can be calculated by comparing the OD of the samples to the standard curve.