Anti-GABARAPL1 Antibody

Rabbit Polyclonal Antibody specific to GABARAPL1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Early estrogen-regulated protein, GABA(A receptor-associated protein-like 1, Glandular epithelial cell protein 1, GEC-1

Gene Name

GABARAPL1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=GABARAPL1">GABARAPL1</a>

UniProt

Q9H0R8

Cellular Locus

Cytoplasmic vesicle, autophagosome, Cytoplasmic vesicle membrane, Cytoplasm, cytoskeleton, Endoplasmic reticulum, Golgi apparatus

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C-terminus of human GABARAPL1

Target Antigen

γ-aminobutyric acid receptor-associated protein-like 1

Target

GABARAPL1

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor (PubMed:16431922). Involved in formation of autophagosomal vacuoles (PubMed:20404487). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20404487). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537). {PubMed:16431922, PubMed:20404487, PubMed:31006537, PubMed:31006538}.

Additionnal Information

Immunoblotting: use at dilution of 1:1,000. A band of ~14-16kDa is detected. Immunofluorescence: use at dilution of 1:100 These are recommended working dilutions. Endusers should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human GABARAPL1.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

GABARAP, GABARAPL1 and GABARAPL2 genes are differentially expressed in normal and pathological tissues. The GABARAP gene is highly expressed in endocrine tissues while the GABARAPL1 gene is predominantly expressed in the central nervous system, but they both are underexpressed in a large variety of cancer cell lines. However, the analysis of GABARAPL1 expression in a cohort of 256 breast adenocarcinoma revealed that a low GABARAPL1 expression was correlated with a high risk of metastasis, in particular for lymph node-positive patients.