Anti-TARPγ2/4/8Stargazin Antibody

Mouse Monoclonal Antibody specific to TaARP2/4/8/Stargazin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Neuronal voltage-gated calcium channel γ-2 subunit, Stargazin, Transmembrane AMPAR regulatory protein γ-2, TARP γ-2

Gene Name

Cacng2

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Cacng2">Cacng2</a>

UniProt

Q71RJ2

Accession Number

NP_445803.1

Cellular Locus

Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Fusion protein corresponding to aa 203-323 (cytoplasmic C-terminus) of rat TARPg2 (accession no. Q71RJ2).

Target Antigen

Voltage-dependent calcium channel γ-2 subunit

Target

TARPγ2/4/8Stargazin

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state. {PubMed:17880894, PubMed:18817736, PubMed:19234459, PubMed:19265014, PubMed:20805473}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Predicted molecular weight is 35-55kDa. <br><br>Positive control: Rat brain lysate. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes mouse and rat TARPg2, TARPg4, and TARPg8. It does not cross-react with TARPg3

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Stargazin belongs to a group of voltage- gated calcium channels that are present in most excitable cells. It appears to stabilize calcium channels in an inactivated (closed) state. Voltage-gated calcium channels exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.