Anti-Hsp40 (Hdj1) Antibody

Mouse Monoclonal Antibody specific to Hsp40

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

DnaJ protein homolog 1, Heat shock 40 kDa protein 1, HSP40, Heat shock protein 40, Human DnaJ protein 1, hDj-1

Gene Name

DNAJB1

Gene ID

3337

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=DNAJB1">DNAJB1</a>

UniProt

P25685

Accession Number

NP_006136.1

Cellular Locus

Cytoplasm, Nucleus, Nucleus, nucleolus

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Hsp40 (Hdj1)

Target Antigen

DnaJ homolog subfamily B member 1

Target

Hsp40 (Hdj1)

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA, IHC, IP

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and rPubMed:24318877, PubMed:9499401}.

Additionnal Information

Immunoblotting: use at 0.5-1ug/ml. A band of ~40 kDa is detected.ELISA <br><br>User should determine optimal concentrations for their application. <br><br>Positive control: HeLa cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Hsp40 (Hdj1). Other species have not been tested. It does not cross-react with Hdj2 or Ydj1.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Members of the human Hsp40/DnaJ protein family feature a J domain (named for the bacterial DnaJ protein). The J domain spans the first 75 N- terminal amino acids and is separated from the C-terminus by a glycine- phenylalanine-rech domain. The Hsp40/DnaJ proteins play many different roles in cellular processes such as folding, translocation, degradation, and assembly of multi-protein complexes. Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding as well as in regulation of Hsp70 function. Hsp40 stimulates ATPase activity of Hsp70 which in turn causes conformational changes in unfolded proteins.