Anti-CDX-2 Antibody

Mouse Monoclonal Antibody specific to CDX-2

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CDX-3, Caudal-type homeobox protein 2

Gene Name

CDX2

NCBI Gene ID

UniProt

Q99626

Cellular Locus

Nucleus

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human CDX-2.

Target Antigen

Homeobox protein CDX-2

Target

CDX-2

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

IHC

Field of Research

Cancer research

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Tris

Function

Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536). {UniProtKB:P43241, UniProtKB:Q04649, PubMed:28473536}.

Additionnal Information

<br><br>Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

Human CDX-2. Reactivity with other species has not been investigated.

Formulation

Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.

Buffer pH

pH 7.3-7.7

Target Background

Homeobox protein CDX-2 is a protein that in humans is encoded by the CDX2 gene. This gene is a member of the caudal-related homeobox transcription factor family that is expressed in the nuclei of intestinal epithelial cells, and, as such, is a useful marker for gastrointestinal carcinoma as well as for determining the origin of gastrointestinal metastatic adenocarcinoma and carcinoids.