Anti-Protein Tyrosine Phosphatase Receptor F (PTPRF) Antibody

Mouse Monoclonal Antibody specific to Protein Tyrosine phosphatase Receptor F (PTPRF)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.1.3.48, Leukocyte common antigen related, LAR

Gene Name

PTPRF

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=PTPRF">PTPRF</a>

UniProt

P10586

Cellular Locus

Membrane; Single-pass type I membrane protein.

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 1315-1607 (cytoplasmic C-terminus) of human PTPRF. This sequence is 97

Target Antigen

Receptor-type tyrosine-protein phosphatase F

Target

Protein Tyrosine Phosphatase Receptor F (PTPRF)

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity.; The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~85kDa is detected which represents the subunit containing transmembrane and intracellular domains. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat PTPRF.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

PTPRF, like other members of the protein tyrosine phosphatase (PTP) family, is a signaling molecule that regulates a variety of cellular processes. PTPRF possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and represents a receptor-type PTP. The extracellular region contains three Ig-like domains and nine non-Ig like domains similar to that of neural-cell adhesion molecule. PTPRF functions in the regulation of epithelial cell-cell contacts at adherents junctions as well as in the control of beta- catenin signaling. An increased expression level of PTPRF has been found in the insulin- responsive tissue of obese, insulin-resistant individuals and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.