Human IL-17E / IL-25 Protein, His Tag (MALS verified)

Interleukin 25 (IL25) is also known as Interleukin-17E (IL-17E), is a distinct member of the IL17 cytokine family comprised of at least six members sharing a conserved cysteine-knot structure but divergent at the N-terminus. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. IL25 can induce NF-κB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17RB. IL-25 induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-25 can kill some types of breast cancer cells.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.8 kDa. The protein migrates as 20-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_073626.1

Expression Region

Tyr 33 - Gly 177

Host

HEK293

Target

IL-17E

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human IL-17 RB, Fc Tag (Cat. No. ILB-H5251) at 5 μg/mL (100 μL/well) can bind Human IL-17E, His Tag (Cat. No. IL5-H52H9) with a linear range of 0.2-3 ng/mL (QC tested).